Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NODSCID versus NODSCIDγ chainnull Mice
Joint Authors
Silver, Jayne M.
Pollok, Karen E.
Bailey, Barbara
Baluyut, Arthur R.
Juliar, Beth E.
Hartwell, Jennifer R.
Sinn, Anthony L.
Cai, Shanbao
Wang, Haiyan
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Humanized bone-marrow xenograft models that can monitor the long-term impact of gene-therapy strategies will help facilitate evaluation of clinical utility.
The ability of the murine bone-marrow microenvironment in NOD/SCID versus NOD/SCID/γ chainnull mice to support long-term engraftment of MGMTP140K-transduced human-hematopoietic cells following alkylator-mediated in vivo selection was investigated.
Mice were transplanted with MGMTP140K-transduced CD34+ cells and transduced cells selected in vivo.
At 4 months after transplantation, levels of human-cell engraftment, and MGMTP140K-transduced cells in the bone marrow of NOD/SCID versus NSG mice varied slightly in vehicle- and drug-treated mice.
In secondary transplants, although equal numbers of MGMTP140K-transduced human cells were transplanted, engraftment was significantly higher in NOD/SCID/γ chainnull mice compared to NOD/SCID mice at 2 months after transplantation.
These data indicate that reconstitution of NOD/SCID/γ chainnull mice with human-hematopoietic cells represents a more promising model in which to test for genotoxicity and efficacy of strategies that focus on manipulation of long-term repopulating cells of human origin.
American Psychological Association (APA)
Cai, Shanbao& Wang, Haiyan& Bailey, Barbara& Hartwell, Jennifer R.& Silver, Jayne M.& Juliar, Beth E.…[et al.]. 2010. Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NODSCID versus NODSCIDγ chainnull Mice. Bone Marrow Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-457618
Modern Language Association (MLA)
Cai, Shanbao…[et al.]. Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NODSCID versus NODSCIDγ chainnull Mice. Bone Marrow Research No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-457618
American Medical Association (AMA)
Cai, Shanbao& Wang, Haiyan& Bailey, Barbara& Hartwell, Jennifer R.& Silver, Jayne M.& Juliar, Beth E.…[et al.]. Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NODSCID versus NODSCIDγ chainnull Mice. Bone Marrow Research. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-457618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457618