Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents andor Histone Deacetylase Inhibitors
Joint Authors
Brodská, Barbora
Holoubek, Aleš
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Reactive oxygen species play an important role in the process of apoptosis in many cell types.
In this paper, we analyzed the role of ROS in DNA-damaging agents (actinomycin D or decitabine), which induced apoptosis of leukemia cell line CML-T1 and normal peripheral blood lymphocytes (PBL).
The possibility of synergism with histone deacetylase inhibitors butyrate or SAHA is also reported.
We found that in cancer cell line, ROS production significantly contributed to apoptosis triggering, while in normal lymphocytes treated by cytostatic or cytotoxic drugs, necrosis as well as apoptosis occurred and large heterogeneity of ROS production was measured.
Combined treatment with histone deacetylase inhibitor did not potentiate actinomycin D action, whereas combination of decitabine and SAHA brought synergistic ROS generation and apoptotic features in CML cell line.
Appropriate decrease of cell viability indicated promising therapeutic potential of this combination in CML, but side effects on normal PBL should be taken into attention.
American Psychological Association (APA)
Brodská, Barbora& Holoubek, Aleš. 2011. Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents andor Histone Deacetylase Inhibitors. Oxidative Medicine and Cellular Longevity،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457666
Modern Language Association (MLA)
Brodská, Barbora& Holoubek, Aleš. Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents andor Histone Deacetylase Inhibitors. Oxidative Medicine and Cellular Longevity No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-457666
American Medical Association (AMA)
Brodská, Barbora& Holoubek, Aleš. Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents andor Histone Deacetylase Inhibitors. Oxidative Medicine and Cellular Longevity. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457666