Nanotomography of Cell Surfaces with Evanescent Fields
Joint Authors
Wagner, Michael
Lassalle, Henri-Pierre
Strauss, Wolfgang S. L.
Schneckenburger, Herbert
Weber, Petra
Source
Advances in Optical Technologies
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-06-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Topics
Abstract EN
The technique of variable-angle total internal reflection fluorescence microscopy (TIRFM) and its application to nanotomography of cell surfaces are described.
Present applications include (1) 3D imaging of chromosomes in their metaphase to demonstrate axial resolution in the nanometre range, (2) measurements of cell-substrate topology, which upon cholesterol depletion shows some loosening of cell-substrate contacts, and (3) measurements of cell topology upon photodynamic therapy (PDT), which demonstrate cell swelling and maintenance of focal contacts.
The potential of the method for in vitro diagnostics, but also some requirements and limitations are discussed.
American Psychological Association (APA)
Wagner, Michael& Weber, Petra& Strauss, Wolfgang S. L.& Lassalle, Henri-Pierre& Schneckenburger, Herbert. 2008. Nanotomography of Cell Surfaces with Evanescent Fields. Advances in Optical Technologies،Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-457750
Modern Language Association (MLA)
Wagner, Michael…[et al.]. Nanotomography of Cell Surfaces with Evanescent Fields. Advances in Optical Technologies No. 2008 (2008), pp.1-7.
https://search.emarefa.net/detail/BIM-457750
American Medical Association (AMA)
Wagner, Michael& Weber, Petra& Strauss, Wolfgang S. L.& Lassalle, Henri-Pierre& Schneckenburger, Herbert. Nanotomography of Cell Surfaces with Evanescent Fields. Advances in Optical Technologies. 2008. Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-457750
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457750
