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Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice
Joint Authors
Chao, Wen-Wan
Kuo, Yueh-Hsiung
Hsieh, Shie-Liang
Lin, Bi-Fong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study was to investigate anti-inflammatory effect of Andrographis paniculata (Burm.
f.) Nees (Acanthaceae) (AP).
The effects of ethyl acetate (EtOAc) extract from AP on the level of inflammatory mediators were examined first using nuclear factor kappa B (NF-κB) driven luciferase assay.
The results showed that AP significantly inhibited NF-κB luciferase activity and tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), macrophage inflammatory protein-2 (MIP-2) and nitric oxide (NO) secretions from lipopolysaccharide (LPS)/interferon-γ stimulated Raw264.7 cells.
To further evaluate the anti-inflammatory effects of AP in vivo, BALB/c mice were tube-fed with 0.78 (AP1), 1.56 (AP2), 3.12 (AP3) and 6.25 (AP4) mg kg−1 body weight (BW)/day in soybean oil, while the control and PDTC (pyrrolidine dithiocarbamate, an anti-inflammatory agent) groups were tube-fed with soybean oil only.
After 1 week of tube-feeding, the PDTC group was injected with 50 mg kg−1 BW PDTC and 1 h later, all of the mice were injected with 15 mg kg−1 BW LPS.
The results showed that the AP1, AP2, AP3 and PDTC groups, but not AP4, had significantly higher survival rate than the control group.
Thus, the control, AP1, AP2, AP3 and PDTC groups were repeated for in vivo parameters.
The results showed that the AP and PDTC groups had significantly lower TNF-α, IL-12p40, MIP-2 or NO in serum or peritoneal macrophages and infiltration of inflammatory cells into the lung of mice.
The AP1 group also had significantly lower MIP-2 mRNA expression in brain.
This study suggests that AP can inhibit the production of inflammatory mediators and alleviate acute hazards at its optimal dosages.
American Psychological Association (APA)
Chao, Wen-Wan& Kuo, Yueh-Hsiung& Hsieh, Shie-Liang& Lin, Bi-Fong. 2011. Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-457768
Modern Language Association (MLA)
Chao, Wen-Wan…[et al.]. Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-457768
American Medical Association (AMA)
Chao, Wen-Wan& Kuo, Yueh-Hsiung& Hsieh, Shie-Liang& Lin, Bi-Fong. Inhibitory Effects of Ethyl Acetate Extract of Andrographis paniculata on NF-κB Trans-Activation Activity and LPS-Induced Acute Inflammation in Mice. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-457768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457768