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Straightforward Procedure for Laboratory Production of DNA Ladder
Joint Authors
Loan, Pham Thi Thanh
Thanh, Le Thi
Lan, Vo Thi Thuong
Duong, Pham Anh Thuy
Thuan, Ta Bich
Ha, Ngo Thi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-12
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories.
In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes.
DNA fragments of 100 bp with unique restriction site at both ends were self-ligated to create a tandem repeat.
Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments.
Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with current ones widely used in most laboratories.
American Psychological Association (APA)
Lan, Vo Thi Thuong& Loan, Pham Thi Thanh& Duong, Pham Anh Thuy& Thanh, Le Thi& Ha, Ngo Thi& Thuan, Ta Bich. 2012. Straightforward Procedure for Laboratory Production of DNA Ladder. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-457777
Modern Language Association (MLA)
Lan, Vo Thi Thuong…[et al.]. Straightforward Procedure for Laboratory Production of DNA Ladder. Journal of Nucleic Acids No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-457777
American Medical Association (AMA)
Lan, Vo Thi Thuong& Loan, Pham Thi Thanh& Duong, Pham Anh Thuy& Thanh, Le Thi& Ha, Ngo Thi& Thuan, Ta Bich. Straightforward Procedure for Laboratory Production of DNA Ladder. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-457777
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457777