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mRNAmicroRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus)
Joint Authors
Xu, Liuxiong
Gong, Xiaoling
Bao, Baolong
Gai, Junwei
Xing, Jubin
Yang, Linlin
Xie, Caixia
Ke, Zhonghe
Xu, Shanliang
Source
Comparative and Functional Genomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Flatfish is famous for the asymmetric transformation during metamorphosis.
The molecular mechanism behind the asymmetric development has been speculated over a century and is still not well understood.
To date, none of the metamorphosis-related genes has been identified in flatfish.
As the first step to screen metamorphosis-related gene, we constructed a whole-body cDNA library and a whole-body miRNA library in this study and identified 1051 unique ESTs, 23 unique miRNAs, and 4 snoRNAs in premetamorphosing and prometamorphosing Paralichthys olivaceus.
1005 of the ESTs were novel, suggesting that there was a special gene expression profile at metamorphic stage.
Four miRNAs (pol-miR-20c, pol-miR-23c, pol-miR-130d, and pol-miR-181e) were novel to P.
olivaceus; they were characterized as highly preserved homologies of published miRNAs but with at least one nucleotide differed.
Representative 24 mRNAs and 23 miRNAs were quantified during metamorphosis of P.
olivaceus by using quantitative RT PCR or stem-loop qRT PCR.
Our results showed that 20 of mRNAs might be associated with early metamorphic events, 10 of mRNAs might be related with later metamorphic events, and 16 of miRNAs might be involved in the regulation of metamorphosis.
The data provided in this study would be helpful for further identifying metamorphosis-related gene in P.
olivaceus.
American Psychological Association (APA)
Xie, Caixia& Xu, Shanliang& Yang, Linlin& Ke, Zhonghe& Xing, Jubin& Gai, Junwei…[et al.]. 2011. mRNAmicroRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus). Comparative and Functional Genomics،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-457822
Modern Language Association (MLA)
Xie, Caixia…[et al.]. mRNAmicroRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus). Comparative and Functional Genomics No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-457822
American Medical Association (AMA)
Xie, Caixia& Xu, Shanliang& Yang, Linlin& Ke, Zhonghe& Xing, Jubin& Gai, Junwei…[et al.]. mRNAmicroRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus). Comparative and Functional Genomics. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-457822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457822