Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm
Joint Authors
Galvão, Lívia Câmara de Carvalho
da Cunha, Marcos Guilherme
Bueno-Silva, Bruno
Ikegaki, Masaharu
de Alencar, Severino Matias
Franchin, Marcelo
Rosalen, Pedro Luiz
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis on Streptococcus mutans biofilm.
The ethanolic extract of Melipona scutellaris geopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity.
The effects of HF on S.
mutans UA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays.
Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM).
HF at 250 μg/mL and 400 μg/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P<0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins.
In addition, the S.
mutans viability (killing assay) and acid production by glycolytic pH drop were not affected (P>0.05).
In conclusion, the bioactive HF of geopropolis was promising to control the S.
mutans biofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.
American Psychological Association (APA)
da Cunha, Marcos Guilherme& Franchin, Marcelo& Galvão, Lívia Câmara de Carvalho& Bueno-Silva, Bruno& Ikegaki, Masaharu& de Alencar, Severino Matias…[et al.]. 2013. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-457853
Modern Language Association (MLA)
da Cunha, Marcos Guilherme…[et al.]. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-457853
American Medical Association (AMA)
da Cunha, Marcos Guilherme& Franchin, Marcelo& Galvão, Lívia Câmara de Carvalho& Bueno-Silva, Bruno& Ikegaki, Masaharu& de Alencar, Severino Matias…[et al.]. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-457853
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457853