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Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms
Joint Authors
Zeng, Zhi-Peng
Yin, Chun-Ling
Du, Ya-Hui
Hu, Le-Qian
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A simple, rapid, and sensitive method for the simultaneous determination of vancomycin and cephalexin in human plasma was developed by using HPLC-DAD with second-order calibration algorithms.
Instead of a completely chromatographic separation, mathematical separation was performed by using two trilinear decomposition algorithms, that is, PARAFAC-alternative least squares (PARAFAC-ALSs) and self-weight-alternative-trilinear-decomposition- (SWATLD-) coupled high-performance liquid chromatography with DAD detection.
The average recoveries attained from PARAFAC-ALS and SWATLD with the factor number of 4 (N=4) were 101±5% and 102±4% for vancomycin, and 96±3% and 97±3% for cephalexininde in real human samples, respectively.
The statistical comparison between PARAFAC-ALS and SWATLD is demonstrated to be similar.
The results indicated that the combination of HPLC-DAD detection with second-order calibration algorithms is a powerful tool to quantify the analytes of interest from overlapped chromatographic profiles for complex analysis of drugs in plasma.
American Psychological Association (APA)
Hu, Le-Qian& Yin, Chun-Ling& Du, Ya-Hui& Zeng, Zhi-Peng. 2012. Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-457915
Modern Language Association (MLA)
Hu, Le-Qian…[et al.]. Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-457915
American Medical Association (AMA)
Hu, Le-Qian& Yin, Chun-Ling& Du, Ya-Hui& Zeng, Zhi-Peng. Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-457915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457915