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In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria
Joint Authors
Abdul Wahab, Norhanom
Abd Malek, Sri Nurestri
Syed Abdul Rahman, Syarifah Nur
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Bioassay-guided isolation of the active hexane fractions of Curcuma zedoaria led to the identification of five pure compounds, namely, curzerenone (1), neocurdione (2), curdione (3), alismol (4), and zederone (5) and a mixture of sterols, namely, campesterol (6), stigmasterol (7), and β-sitosterol (8).
Alismol has never been reported to be present in Curcuma zedoaria.
All isolated compounds except (3) were evaluated for their cytotoxic activity against MCF-7, Ca Ski, and HCT-116 cancer cell lines and noncancer human fibroblast cell line (MRC-5) using neutral red cytotoxicity assay.
Curzerenone and alismol significantly inhibited cell proliferation in human cancer cell lines MCF-7, Ca Ski, and HCT-116 in a dose-dependent manner.
Cytological observations by an inverted phase contrast microscope and Hoechst 33342/PI dual-staining assay showed typical apoptotic morphology of cancer cells upon treatment with curzerenone and alismol.
Both compounds induce apoptosis through the activation of caspase-3.
It can thus be suggested that curzerenone and alismol are modulated by apoptosis via caspase-3 signalling pathway.
The findings of the present study support the use of Curcuma zedoaria rhizomes in traditional medicine for the treatment of cancer-related diseases.
Thus, two naturally occurring sesquiterpenoids, curzerenone and alismol, hold great promise for use in chemopreventive and chemotherapeutic strategies.
American Psychological Association (APA)
Syed Abdul Rahman, Syarifah Nur& Abdul Wahab, Norhanom& Abd Malek, Sri Nurestri. 2013. In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-457937
Modern Language Association (MLA)
Syed Abdul Rahman, Syarifah Nur…[et al.]. In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-457937
American Medical Association (AMA)
Syed Abdul Rahman, Syarifah Nur& Abdul Wahab, Norhanom& Abd Malek, Sri Nurestri. In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-457937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457937