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Evaluation of BACE1 Silencing in Cellular Models
Joint Authors
Kuwabara, Tomoko
Nawrot, Barbara
Kazmierczak-Baranska, Julia
Kubiak, Katarzyna
Sierant, Malgorzata
Warashina, Masaki
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Beta-secretase (BACE1) is the major enzyme participating in generation of toxic amyloid-beta (Aβ) peptides, identified in amyloid plaques of Alzheimer's disease (AD) brains.
Its downregulation results in decreasing secretion of Aβ.
Thus, BACE1 silencing by RNAi represents possible strategy for antiamyloid therapy in the treatment of AD.
In this study, a series of newly designed sequences of synthetic and vector-encoded siRNAs (pSilencer, pcPURhU6, and lentivirus) were tested against overexpressed and endogenous BACE1 in several cell lines and in adult neural progenitor cells, derived from rat hippocampus.
SiRNAs active in human, mouse, and rat cell models were shown to diminish the level of BACE1.
In HCN A94 cells, two BACE1-specific siRNAs did not alter the expression of genes of BACE2 and several selected genes involved in neurogenesis (Synapsin I, βIII-Tubulin, Calbidin, NeuroD1, GluR2, CREB, MeCP2, PKR), however, remarkable lowering of SCG10 mRNA, coding protein of stathmin family, important in the development of nervous system, was observed.
American Psychological Association (APA)
Sierant, Malgorzata& Kubiak, Katarzyna& Kazmierczak-Baranska, Julia& Warashina, Masaki& Kuwabara, Tomoko& Nawrot, Barbara. 2009. Evaluation of BACE1 Silencing in Cellular Models. International Journal of Alzheimer's Disease،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-457964
Modern Language Association (MLA)
Sierant, Malgorzata…[et al.]. Evaluation of BACE1 Silencing in Cellular Models. International Journal of Alzheimer's Disease No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-457964
American Medical Association (AMA)
Sierant, Malgorzata& Kubiak, Katarzyna& Kazmierczak-Baranska, Julia& Warashina, Masaki& Kuwabara, Tomoko& Nawrot, Barbara. Evaluation of BACE1 Silencing in Cellular Models. International Journal of Alzheimer's Disease. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-457964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457964