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Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats
Joint Authors
Wei, Jia
Chen, Jian-Guo
Hu, Chang-Min
Cheng, Li-Li
Wang, Qiong
Wu, Yi-Qiang
Shah, Zahir
Ding, Ming-Xing
Liu, Dong-Ming
Qiu, Zheng-Ying
Hu, Man-Li
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In order to investigate the dynamic processes of mRNA levels of proenkephalin, proopiomelanocortin, prodynorphin, and opioid receptors (δ-, μ-, and κ-receptor) induced by electroacupuncture (EA) in the central nerve system, goats were stimulated by EA of 60 Hz for 0.5 h at a set of Baihui, Santai, Ergen, and Sanyangluo points.
The pain threshold was measured using the method of potassium iontophoresis.
The mRNA levels of the three opioid peptide precursors and three opioid receptors were determined with quantitative real-time PCR and the levels of Met-enkephalin with SABC immunohistochemistry at 0.5 h before and at 0, 2, 4, 6, 8, 12, and 24 h after EA.
The results showed that the pain threshold correlated (P<0.01) with Met-enkephalin immunoactivities in the measured nuclei and areas of goats.
The analgesic aftereffect lasted for 12 h at least.
The mRNA levels of the three opioid peptide precursors and three opioid receptors began to increase at 0 h, reached the peak during the time from 4 h to 6 h or at 12 h, and remained higher at 24 h after EA was discontinued.
These results suggested that the initiation of gene expression of opioid peptides and the three receptors may be associated with EA-induced analgesic aftereffect.
American Psychological Association (APA)
Cheng, Li-Li& Ding, Ming-Xing& Wei, Jia& Wu, Yi-Qiang& Qiu, Zheng-Ying& Chen, Jian-Guo…[et al.]. 2013. Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-457993
Modern Language Association (MLA)
Cheng, Li-Li…[et al.]. Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-457993
American Medical Association (AMA)
Cheng, Li-Li& Ding, Ming-Xing& Wei, Jia& Wu, Yi-Qiang& Qiu, Zheng-Ying& Chen, Jian-Guo…[et al.]. Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-457993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457993