A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets
Joint Authors
Munib-ur-Rehman,
Yousuf, Rabia Ismail
Shoaib, Muhammad Harris
Source
Chromatography Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Stability indicating reversed phase HPLC method was developed and validated for the simultaneous quantitation of antitubercular drugs, ethionamide (ETH), and moxifloxacin (MOX) with commonly coprescribed vitamin, pyridoxine (PYR) in tablet dosage form.
The method was found rapid, precise and accurate.
The separation was performed in Hibar 150-4.6, Purospher STAR, RP-18e (5 μm) column, using mobile phase A (0.03 M sodium citrate adjusted to pH 5 with glacial acetic acid) and mobile phase B (100% methanol), ran at variable proportions at flow rate of 1.0 mL/min.
The detection was carried out at 320 nm.
The method was observed linearly in the range of 2.5–17.5 μg/mL for PYR, 25–175 μg/mL for ETH, and 40–280 μg/mL for MOX with respective limits of detection/quantitation of 0.125 μg/mL/1.28 μg/mL, 0.25 μg/mL/2.56 μg/mL, and 0.35 μg/mL/3.65 μg/mL.
The drugs were also subjected to oxidative, hydrolytic, photolytic, and thermal degradation; the degradation products showed interference with the detection of PYR, ETH, and MOX.
The proposed method was observed to be effective to quantitate MOX (400 mg), ETH (250 mg), and PYR (25 mg) in fixed dose combination tablet formulation.
American Psychological Association (APA)
Munib-ur-Rehman, & Yousuf, Rabia Ismail& Shoaib, Muhammad Harris. 2014. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458048
Modern Language Association (MLA)
Munib-ur-Rehman,…[et al.]. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-458048
American Medical Association (AMA)
Munib-ur-Rehman, & Yousuf, Rabia Ismail& Shoaib, Muhammad Harris. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458048