A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets

Joint Authors

Munib-ur-Rehman,
Yousuf, Rabia Ismail
Shoaib, Muhammad Harris

Source

Chromatography Research International

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-04-23

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Chemistry

Abstract EN

Stability indicating reversed phase HPLC method was developed and validated for the simultaneous quantitation of antitubercular drugs, ethionamide (ETH), and moxifloxacin (MOX) with commonly coprescribed vitamin, pyridoxine (PYR) in tablet dosage form.

The method was found rapid, precise and accurate.

The separation was performed in Hibar 150-4.6, Purospher STAR, RP-18e (5 μm) column, using mobile phase A (0.03 M sodium citrate adjusted to pH 5 with glacial acetic acid) and mobile phase B (100% methanol), ran at variable proportions at flow rate of 1.0 mL/min.

The detection was carried out at 320 nm.

The method was observed linearly in the range of 2.5–17.5 μg/mL for PYR, 25–175 μg/mL for ETH, and 40–280 μg/mL for MOX with respective limits of detection/quantitation of 0.125 μg/mL/1.28 μg/mL, 0.25 μg/mL/2.56 μg/mL, and 0.35 μg/mL/3.65 μg/mL.

The drugs were also subjected to oxidative, hydrolytic, photolytic, and thermal degradation; the degradation products showed interference with the detection of PYR, ETH, and MOX.

The proposed method was observed to be effective to quantitate MOX (400 mg), ETH (250 mg), and PYR (25 mg) in fixed dose combination tablet formulation.

American Psychological Association (APA)

Munib-ur-Rehman, & Yousuf, Rabia Ismail& Shoaib, Muhammad Harris. 2014. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458048

Modern Language Association (MLA)

Munib-ur-Rehman,…[et al.]. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-458048

American Medical Association (AMA)

Munib-ur-Rehman, & Yousuf, Rabia Ismail& Shoaib, Muhammad Harris. A Stability-Indicating High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Pyridoxine, Ethionamide, and Moxifloxacin in Fixed Dose Combination Tablets. Chromatography Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458048

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-458048