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Effects of Parathyroid Hormone on Calcium Ions in Rat Bone Marrow Mesenchymal Stem Cells
Joint Authors
Zeng, Yanjun
Chen, Yushu
Chen, Yi
Bai, Bo
Ye, Jing
Zhang, Shujiang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The present study was conducted in order to explore the mechanisms whereby parathyroid hormone (PTH) maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs).
Bone marrow was isolated from Sprague Dawley (SD) rat femurs, cultured in vitro, and passaged using a cell adherent culture method.
The BMSC proliferation was evaluated by the methyl thiazolyl tetrazolium (MTT) assay and the fluorescence intensity of calcium ions in BMSCs was analyzed by laser scanning confocal microscopy (LSCM).
Our results show that BMSC proliferation in the experimental group treated with PTH was more significant than controls.
The calcium ion fluorescence intensity in BMSCs was significantly higher for the experimental group as compared to the control group.
For each group, there was significant difference in the fluorescence intensity of calcium ions in BMSCs between 7 d and 14 d.
In conclusion, parathyroid hormone increased the fluorescence intensity of calcium ions in BMSCs, which might represent a key mechanism whereby BMSC proliferation is maintained.
American Psychological Association (APA)
Chen, Yushu& Bai, Bo& Zhang, Shujiang& Ye, Jing& Chen, Yi& Zeng, Yanjun. 2014. Effects of Parathyroid Hormone on Calcium Ions in Rat Bone Marrow Mesenchymal Stem Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-458082
Modern Language Association (MLA)
Chen, Yushu…[et al.]. Effects of Parathyroid Hormone on Calcium Ions in Rat Bone Marrow Mesenchymal Stem Cells. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-458082
American Medical Association (AMA)
Chen, Yushu& Bai, Bo& Zhang, Shujiang& Ye, Jing& Chen, Yi& Zeng, Yanjun. Effects of Parathyroid Hormone on Calcium Ions in Rat Bone Marrow Mesenchymal Stem Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-458082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458082