Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2 : A Growth Factor to Induce Dentin Regeneration
Joint Authors
Nishihara, Tatsuji
Kitamura, Chiaki
Morotomi, Takahiko
Washio, Ayako
Terashita, Masamichi
Source
International Journal of Dentistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process.
After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined.
BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7.
BMP-2 also induced the expressions of DSP and DMP-1.
Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways.
American Psychological Association (APA)
Washio, Ayako& Kitamura, Chiaki& Morotomi, Takahiko& Terashita, Masamichi& Nishihara, Tatsuji. 2012. Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2 : A Growth Factor to Induce Dentin Regeneration. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458089
Modern Language Association (MLA)
Washio, Ayako…[et al.]. Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2 : A Growth Factor to Induce Dentin Regeneration. International Journal of Dentistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-458089
American Medical Association (AMA)
Washio, Ayako& Kitamura, Chiaki& Morotomi, Takahiko& Terashita, Masamichi& Nishihara, Tatsuji. Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2 : A Growth Factor to Induce Dentin Regeneration. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458089
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458089