Antibodies Induced by Lipoarabinomannan in Bovines : Characterization and Effects on the Interaction between Mycobacterium avium Subsp. paratuberculosis and Macrophages In Vitro
Joint Authors
Fernández, Eloy
Fernández, Bárbara
Jolly, Ana
Mundo, Silvia Leonor
Colavecchia, Silvia Beatriz
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lipoarabinomannan (LAM) is a major glycolipidic antigen on the mycobacterial envelope.
The aim of this study was to characterize the humoral immune response induced by immunization with a LAM extract in bovines and to evaluate the role of the generated antibodies in the in vitro infection of macrophages with Mycobacterium avium subsp.
paratuberculosis (MAP).
Sera from fourteen calves immunized with LAM extract or PBS emulsified in Freund's Incomplete Adjuvant and from five paratuberculosis-infected bovines were studied.
LAM-immunized calves developed specific antibodies with IgG1 as the predominant isotype.
Serum immunoglobulins were isolated and their effect was examined in MAP ingestion and viability assays using a bovine macrophage cell line.
Our results show that the antibodies generated by LAM immunization significantly increase MAP ingestion and reduce its intracellular viability, suggesting an active role in this model.
American Psychological Association (APA)
Jolly, Ana& Colavecchia, Silvia Beatriz& Fernández, Bárbara& Fernández, Eloy& Mundo, Silvia Leonor. 2011. Antibodies Induced by Lipoarabinomannan in Bovines : Characterization and Effects on the Interaction between Mycobacterium avium Subsp. paratuberculosis and Macrophages In Vitro. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-458092
Modern Language Association (MLA)
Jolly, Ana…[et al.]. Antibodies Induced by Lipoarabinomannan in Bovines : Characterization and Effects on the Interaction between Mycobacterium avium Subsp. paratuberculosis and Macrophages In Vitro. Veterinary Medicine International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-458092
American Medical Association (AMA)
Jolly, Ana& Colavecchia, Silvia Beatriz& Fernández, Bárbara& Fernández, Eloy& Mundo, Silvia Leonor. Antibodies Induced by Lipoarabinomannan in Bovines : Characterization and Effects on the Interaction between Mycobacterium avium Subsp. paratuberculosis and Macrophages In Vitro. Veterinary Medicine International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-458092
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458092