Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages
Joint Authors
Gibbs, Paul A.
Amorim, Lara R. P.
Silva, Joana G. L.
Teixeira, Paula C.
Source
International Journal of Microbiology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp.
by using specific agents.
Three bacteriophages and twelve strains of Salmonella spp.
were tested.
Each of the twelve strains was used separately to inoculate TSB together with each one of the phages.
The inoculum concentration was between 106 and 107 cfu/mL, at a cell: phage ratio of 1 : 100.
From the sample analysis, based on conductance (G) measurements (37°C), the infection could be detected, by observation of both detection-time delay and distinct curve trends.
The main conclusions were that kinetic detection by impedance microbiology with phage typing constitutes a method of determining whether a test microorganism is sensitive to the bacteriophage and a method to evaluate whether a lytic bacteriophage is present in a sample, by affecting bacterial growth rate/metabolic change.
American Psychological Association (APA)
Amorim, Lara R. P.& Silva, Joana G. L.& Gibbs, Paul A.& Teixeira, Paula C.. 2009. Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages. International Journal of Microbiology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-458189
Modern Language Association (MLA)
Amorim, Lara R. P.…[et al.]. Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages. International Journal of Microbiology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-458189
American Medical Association (AMA)
Amorim, Lara R. P.& Silva, Joana G. L.& Gibbs, Paul A.& Teixeira, Paula C.. Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages. International Journal of Microbiology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-458189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458189