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The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition
Joint Authors
Ximenes, Valdecir Farias
dos Santos Vilela, Maria Marluce
Condino-Neto, Antonio
de Almeida, Ana Carolina
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Apocynin is widely used as an inhibitor of the NADPH oxidase.
Since myeloperoxidase (MPO) has been considered as essential for the mechanism of action of apocynin, here we used cells with different levels of MPO and compared their sensitivity to apocynin.
HL-60 cells were differentiated with DMSO or IFNγ/TNFα and compared with peripheral mononuclear (PBMC) and polymorphonuclear cells (PMN).
The relative MPO activity was PBMC = HL60 DMSO < HL60 IFNγ < PMN.
Apocynin inhibited the intracellular reactive oxygen species production by PMN (80%) and IFNγ/TNFα-differentiated HL-60 cells (45%) but showed a minor effect in PBMC and DMSO differentiated HL-60 cells (20%).
The addition of azide decreased the efficiency of apocynin in PMN and the addition of peroxidase increased the inhibition in PBMC.
We also determined the gene expression of the components gp91phox, p47phox, p22phox and p67phox in the resting cells.
Apocynin did not change gp91phox, p47phox or p22phox gene expression in nonstimulated PBMC, HL60 DMSO, HL60 IFNγ/TNFα, and PMN and has a subtle increase in p67phox in HL60 IFNγ/TNFα.
The results from this work suggest that a rational search for better inhibitors of NADPH oxidase in leukocytes should include a correlation with their affinity as substrates for MPO.
American Psychological Association (APA)
de Almeida, Ana Carolina& dos Santos Vilela, Maria Marluce& Condino-Neto, Antonio& Ximenes, Valdecir Farias. 2012. The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition. ISRN Inflammation،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-458267
Modern Language Association (MLA)
de Almeida, Ana Carolina…[et al.]. The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition. ISRN Inflammation No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-458267
American Medical Association (AMA)
de Almeida, Ana Carolina& dos Santos Vilela, Maria Marluce& Condino-Neto, Antonio& Ximenes, Valdecir Farias. The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition. ISRN Inflammation. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-458267
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458267