Berberine Protects against Palmitate-Induced Endothelial Dysfunction : Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
Joint Authors
Li, Ji
Chen, Li
Li, Yu-Lin
Li, Jing
Zhang, Ming
Wei, Sheng-Nan
Bucala, Richard
Wang, Chun-Mei
Meng, Zhao-Jie
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Endothelial dysfunction is a critical factor during the initiation of cardiovascular complications in diabetes.
Berberine can ameliorate endothelial dysfunction induced by diabetes.
However, the underlying mechanisms remain unclear.
The aim of this study was to investigate the protective effect and mechanism of berberine on palmitate-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs).
The cell viability of HUVECs was determined by MTT assays.
Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs.
The mRNA level of endothelial nitric oxide synthase (eNOS) was measured by RT-PCR, and the protein levels of eNOS, p-eNOS, Akt, p-Akt, AMPK, p-AMPK, and NADPH oxidase (NOX4) were analyzed.
The results demonstrated that berberine significantly elevated NO levels and reduced the production of ROS.
The expressions of eNOS were significantly increased, while NOX4 protein expression was decreased in berberine-treated HUVECs.
Moreover, berberine upregulated the protein expression of AMPK and p-AMPK in palmitate-treated HUVECs, but had no effect on the levels of Akt.
Therefore, berberine ameliorates palmitate-induced endothelial dysfunction by upregulating eNOS expression and downregulating expression of NOX4.
This regulatory effect of berberine may be related to the activation of AMPK.
American Psychological Association (APA)
Zhang, Ming& Wang, Chun-Mei& Li, Jing& Meng, Zhao-Jie& Wei, Sheng-Nan& Li, Ji…[et al.]. 2013. Berberine Protects against Palmitate-Induced Endothelial Dysfunction : Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-458269
Modern Language Association (MLA)
Zhang, Ming…[et al.]. Berberine Protects against Palmitate-Induced Endothelial Dysfunction : Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-458269
American Medical Association (AMA)
Zhang, Ming& Wang, Chun-Mei& Li, Jing& Meng, Zhao-Jie& Wei, Sheng-Nan& Li, Ji…[et al.]. Berberine Protects against Palmitate-Induced Endothelial Dysfunction : Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-458269
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458269