![](/images/graphics-bg.png)
Effect of Fluorescent Particle Size on the Modulation Efficiency of Ultrasound-Modulated Fluorescence
Joint Authors
Vignola, Joseph
Yuan, Baohong
Liu, Yuan
Source
International Journal of Optics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To investigate whether the size of fluorescent particles affects the modulation efficiency of ultrasound-modulated fluorescence (UMF), we measured UMF and DC (direct current) signals of the fluorescence emission from four different-sized fluorescent particles: (1) three carboxylate-modified fluorescent microspheres (FM) with diameters of 20 nm, 200 nm, and 1.0 μm and (2) streptavidin-conjugated Alexa Fluor 647 with a diameter of approximately 5 nm.
The UMF and DC signals were simultaneously measured using a broadband lock-in amplifier and a narrowband amplifier, respectively.
The ratio of the UMF strength to the DC signal strength is defined as the modulation efficiency.
This modulation efficiency was then used to evaluate the effects of fluorophore size and concentration.
Results show that the modulation efficiency was improved by approximately a factor of two when the size of the fluorescent particles is increased from 5 nm to 1 μm.
In addition, the linear relationship between the UMF strength and ultrasound pressure (observed in our previous study) was maintained regardless of the fluorescent particle sizes.
American Psychological Association (APA)
Liu, Yuan& Yuan, Baohong& Vignola, Joseph. 2011. Effect of Fluorescent Particle Size on the Modulation Efficiency of Ultrasound-Modulated Fluorescence. International Journal of Optics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-458290
Modern Language Association (MLA)
Liu, Yuan…[et al.]. Effect of Fluorescent Particle Size on the Modulation Efficiency of Ultrasound-Modulated Fluorescence. International Journal of Optics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-458290
American Medical Association (AMA)
Liu, Yuan& Yuan, Baohong& Vignola, Joseph. Effect of Fluorescent Particle Size on the Modulation Efficiency of Ultrasound-Modulated Fluorescence. International Journal of Optics. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-458290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458290