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Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173
Joint Authors
Yadav, Kapil Deo Singh
Yadava, Sudha
Yadav, Meera
Singh, Sunil Kumar
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Mn peroxidase has been purified to homogeneity from the culture filtrate of a new fungal strain Fomes durissimus MTCC-1173 using concentration by ultrafiltration and anion exchange chromatography on diethylaminoethyl (DEAE) cellulose.
The molecular mass of the purified enzyme has been found to be 42.0 kDa using SDS-PAGE analysis.
The Km values using MnSO4 and H2O2 as the variable substrates in 50 mM lactic acid-sodium lactate buffer pH 4.5 at 30°C were 59 μM and 32 μM, respectively.
The catalytic rate constants using MnSO4 and H2O2 were 22.4 s−1 and 14.0 s−1, respectively, giving the values of kcat/Km 0.38 μM−1s−1 and 0.44 μM−1s−1, respectively.
The pH and temperature optima of the Mn peroxidase were 4 and 26°C, respectively.
The purified MnP depolymerises humic acid in presence of H2O2.
The purified Mn peroxidase exhibits haloperoxidase activity at low pH.
American Psychological Association (APA)
Singh, Sunil Kumar& Yadav, Meera& Yadava, Sudha& Yadav, Kapil Deo Singh. 2011. Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173. Bioinorganic Chemistry and Applications،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-458298
Modern Language Association (MLA)
Singh, Sunil Kumar…[et al.]. Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173. Bioinorganic Chemistry and Applications No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-458298
American Medical Association (AMA)
Singh, Sunil Kumar& Yadav, Meera& Yadava, Sudha& Yadav, Kapil Deo Singh. Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173. Bioinorganic Chemistry and Applications. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-458298
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458298