tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii
Joint Authors
Künzi, Andrea
Gebetsberger, Jennifer
Polacek, Norbert
Zywicki, Marek
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nonprotein coding RNA (ncRNA) molecules have been recognized recently as major contributors to regulatory networks in controlling gene expression in a highly efficient manner.
These RNAs either originate from their individual transcription units or are processing products from longer precursor RNAs.
For example, tRNA-derived fragments (tRFs) have been identified in all domains of life and represent a growing, yet functionally poorly understood, class of ncRNA candidates.
Here we present evidence that tRFs from the halophilic archaeon Haloferax volcanii directly bind to ribosomes.
In the presented genomic screen of the ribosome-associated RNome, a 26-residue-long fragment originating from the 5′ part of valine tRNA was by far the most abundant tRF.
The Val-tRF is processed in a stress-dependent manner and was found to primarily target the small ribosomal subunit in vitro and in vivo.
As a consequence of ribosome binding, Val-tRF reduces protein synthesis by interfering with peptidyl transferase activity.
Therefore this tRF functions as ribosome-bound small ncRNA capable of regulating gene expression in H.
volcanii under environmental stress conditions probably by fine tuning the rate of protein production.
American Psychological Association (APA)
Gebetsberger, Jennifer& Zywicki, Marek& Künzi, Andrea& Polacek, Norbert. 2012. tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii. Archaea،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-458307
Modern Language Association (MLA)
Gebetsberger, Jennifer…[et al.]. tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii. Archaea No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-458307
American Medical Association (AMA)
Gebetsberger, Jennifer& Zywicki, Marek& Künzi, Andrea& Polacek, Norbert. tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii. Archaea. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-458307
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458307