RASSF1A and the Taxol Response in Ovarian Cancer
Joint Authors
Kassler, Susannah
Birrer, Michael J.
Donninger, Howard
Clark, Geoffrey J.
Source
Molecular Biology International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The RASSF1A tumor suppressor gene is frequently inactivated by promoter methylation in human tumors.
The RASSF1A protein forms an endogenous complex with tubulin and promotes the stabilization of microtubules.
Loss of RASSF1A expression sensitizes cells to microtubule destabilizing stimuli.
We have observed a strong correlation between the loss of RASSF1A expression and the development of Taxol resistance in primary ovarian cancer samples.
Thus, we sought to determine if RASSF1A levels could dictate the response to Taxol and whether an epigenetic therapy approach might be able to reverse the Taxol resistant phenotype of RASSF1A negative ovarian tumor cells.
We found that knocking down RASSF1A expression in an ovarian cancer cell line inhibited Taxol-mediated apoptosis and promoted cell survival during Taxol treatment.
Moreover, using a combination of small molecule inhibitors of DNA Methyl Transferase enzymes, we were able restore RASSF1A expression and Taxol sensitivity.
This identifies a role for RASSF1A in modulating the tumor response to Taxol and provides proof of principal for the use of epigenetic therapy to overcome Taxol resistance.
American Psychological Association (APA)
Kassler, Susannah& Donninger, Howard& Birrer, Michael J.& Clark, Geoffrey J.. 2012. RASSF1A and the Taxol Response in Ovarian Cancer. Molecular Biology International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458525
Modern Language Association (MLA)
Kassler, Susannah…[et al.]. RASSF1A and the Taxol Response in Ovarian Cancer. Molecular Biology International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-458525
American Medical Association (AMA)
Kassler, Susannah& Donninger, Howard& Birrer, Michael J.& Clark, Geoffrey J.. RASSF1A and the Taxol Response in Ovarian Cancer. Molecular Biology International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-458525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458525