Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson’s Disease Rat Model
Joint Authors
Zheng, Deyu
Jiang, Xiaohua
Zhao, Huanying
Duan, Deyi
Xu, Qunyuan
Zhao, Junpeng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of the present study is to detect a combination method to utilize gene therapy for the treatment of Parkinson’s disease (PD).
Here, a PD rat model is used for the in vivo gene therapy of a recombinant adeno-associated virus (AAV2) containing a human glutamic acid decarboxylase 65 (rAAV2-hGAD65) gene delivered to the subthalamic nucleus (STN).
This is combined with the ex vivo gene delivery of tyrosine hydroxylase (TH) by fibroblasts injected into the striatum.
After the treatment, the rotation behavior was improved with the greatest efficacy in the combination group.
The results of immunohistochemistry showed that hGAD65 gene delivery by AAV2 successfully led to phenotypic changes of neurons in STN.
And the levels of glutamic acid and GABA in the internal segment of the globus pallidus (GPi) and substantia nigra pars reticulata (SNr) were obviously lower than the control groups.
However, hGAD65 gene transfer did not effectively protect surviving dopaminergic neurons in the SNc and VTA.
This study suggests that subthalamic hGAD65 gene therapy and combined with TH gene therapy can alleviate symptoms of the PD model rats, independent of the protection the DA neurons from death.
American Psychological Association (APA)
Zheng, Deyu& Jiang, Xiaohua& Zhao, Junpeng& Duan, Deyi& Zhao, Huanying& Xu, Qunyuan. 2013. Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson’s Disease Rat Model. Neural Plasticity،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-458528
Modern Language Association (MLA)
Zheng, Deyu…[et al.]. Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson’s Disease Rat Model. Neural Plasticity No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-458528
American Medical Association (AMA)
Zheng, Deyu& Jiang, Xiaohua& Zhao, Junpeng& Duan, Deyi& Zhao, Huanying& Xu, Qunyuan. Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson’s Disease Rat Model. Neural Plasticity. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-458528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458528