Transcriptional Regulation of the rsbV Promoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation in Bacillus subtilis
Joint Authors
Choi, Soo-Keun
Saier, Milton H.
Source
International Journal of Microbiology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
The σB-dependent promoter in front of the rsbV gene of Bacillus subtilis is induced ∼5-fold in response to (1) the addition of 4% ethanol, (2) carbon starvation, and (3) phosphorous starvation.
Binding sites for the global carbon and nitrogen regulators, CcpA and TnrA, were mutated, and the consequences of their loss and that of CcpA or TnrA were studied using rsbV-lacZ fusions.
These responses proved to be dependent on CcpA, TnrA, and their putative binding sites upstream of the promoter.
Induction in response to glucose limitation was largely abolished by loss of CcpA or the upstream region, while induction in response to phosphorous limitation was largely abolished only by the upstream mutations.
The results suggest that CcpA directly influences the carbon starvation response and that both proteins exert indirect effects on all three stress responses.
The integrity of the DNA sequence is important for all three responses.
American Psychological Association (APA)
Choi, Soo-Keun& Saier, Milton H.. 2010. Transcriptional Regulation of the rsbV Promoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation in Bacillus subtilis. International Journal of Microbiology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458530
Modern Language Association (MLA)
Choi, Soo-Keun& Saier, Milton H.. Transcriptional Regulation of the rsbV Promoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation in Bacillus subtilis. International Journal of Microbiology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-458530
American Medical Association (AMA)
Choi, Soo-Keun& Saier, Milton H.. Transcriptional Regulation of the rsbV Promoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation in Bacillus subtilis. International Journal of Microbiology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458530
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458530