The Use of Recombinant Pseudotype Virus-Like Particles Harbouring Inserted Target Antigen to Generate Antibodies against Cellular Marker p16INK4A
Joint Authors
Simanaviciene, Vaida
Zvirbliene, Aurelija
Sezaite, Indre
Shikova, Evelina
Gedvilaite, Alma
Norkiene, Milda
Dekaminaviciute, Dovile
Lasickienė, Rita
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Protein engineering provides an opportunity to generate new immunogens with desired features.
Previously, we have demonstrated that hamster polyomavirus major capsid protein VP1-derived virus-like particles (VLPs) are highly immunogenic and can be employed for the insertion of foreign epitopes at certain surface-exposed positions.
In the current study, we have designed pseudotype VLPs consisting of an intact VP1 protein and VP2 protein fused with the target antigen—cellular marker p16INK4A—at its N terminus.
Both proteins coexpressed in yeast were self-assembled to pseudotype VLPs harbouring the inserted antigen on the surface.
The pseudotype VLPs were used for generation of antibodies against p16INK4A that represents a potential biomarker for cells transformed by high-risk human papillomavirus (HPV).
The pseudotype VLPs induced in immunized mice a strong immune response against the target antigen.
The antisera raised against pseudotype VLPs showed specific immunostaining of p16INK4A protein in malignant cervical tissue.
Spleen cells of the immunized mice were used to generate monoclonal antibodies against p16INK4A protein.
The specificity of antibodies was proven by the immunostaining of HPV-transformed cells.
In conclusion, the current study demonstrates the potential of pseudotype VLPs with inserted target antigen as a new type of immunogens to generate antibodies of high diagnostic value.
American Psychological Association (APA)
Lasickienė, Rita& Gedvilaite, Alma& Norkiene, Milda& Simanaviciene, Vaida& Sezaite, Indre& Dekaminaviciute, Dovile…[et al.]. 2012. The Use of Recombinant Pseudotype Virus-Like Particles Harbouring Inserted Target Antigen to Generate Antibodies against Cellular Marker p16INK4A. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458563
Modern Language Association (MLA)
Lasickienė, Rita…[et al.]. The Use of Recombinant Pseudotype Virus-Like Particles Harbouring Inserted Target Antigen to Generate Antibodies against Cellular Marker p16INK4A. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-458563
American Medical Association (AMA)
Lasickienė, Rita& Gedvilaite, Alma& Norkiene, Milda& Simanaviciene, Vaida& Sezaite, Indre& Dekaminaviciute, Dovile…[et al.]. The Use of Recombinant Pseudotype Virus-Like Particles Harbouring Inserted Target Antigen to Generate Antibodies against Cellular Marker p16INK4A. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458563