Modulation of Murine Macrophage TLR78-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium
Joint Authors
Namkoong, Ho
Fujii, Hideki
Tasaka, Sadatomo
Matsushita, Kenichi
Funatsu, Yohei
Asami, Takahiro
Ishii, Ken
Yagi, Kazuma
Betsuyaku, Tomoko
Fujiwara, Hiroshi
Hasegawa, Naoki
Ishii, Makoto
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Increasing evidence suggests that mesenchymal stem cells (MSCs) play anti-inflammatory roles during innate immune responses.
However, little is known about the effect of MSCs or their secretions on the ligand response of Toll-like receptor (TLR) 7 and TLR8, receptors that recognize viral single-stranded RNA (ssRNA).
Macrophages play a critical role in the innate immune response to ssRNA virus infection; therefore, we investigated the effect of MSC-conditioned medium on cytokine expression in macrophages following stimulation with TLR7/8 ligands.
After stimulation with TLR7/8 ligand, bone marrow-derived macrophages cultured with MSCs or in MSC-conditioned medium expressed lower levels of tumor necrosis factor (TNF) α and interleukin (IL) 6 and higher levels of IL-10 compared to macrophages cultured without MSCs or in control medium, respectively.
The modulations of cytokine expression were associated with prostaglandin E2 (PGE2) secreted by the MSCs.
PGE2 enhanced extracellular signal-related kinase (ERK) signaling and suppressed nuclear factor-κB (NF-κB) signaling.
Enhanced ERK signaling contributed to enhanced IL-10 production, and suppression of NF-κB signaling contributed to the low production of TNF-α.
Collectively, these results indicate that MSCs and MSC-conditioned medium modulate the cytokine expression profile in macrophages following TLR7/8-mediated stimulation, which suggests that MSCs play an immunomodulatory role during ssRNA virus infection.
American Psychological Association (APA)
Asami, Takahiro& Ishii, Makoto& Fujii, Hideki& Namkoong, Ho& Tasaka, Sadatomo& Matsushita, Kenichi…[et al.]. 2013. Modulation of Murine Macrophage TLR78-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-458638
Modern Language Association (MLA)
Asami, Takahiro…[et al.]. Modulation of Murine Macrophage TLR78-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium. Mediators of Inflammation No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-458638
American Medical Association (AMA)
Asami, Takahiro& Ishii, Makoto& Fujii, Hideki& Namkoong, Ho& Tasaka, Sadatomo& Matsushita, Kenichi…[et al.]. Modulation of Murine Macrophage TLR78-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-458638
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458638