In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.
Joint Authors
Srivastava, Nidhi
Sharma, Vikas
Kamal, Barkha
Jadon, Vikash Singh
Dobriyal, Anoop Kumar
Source
Biotechnology Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Botany
Agriculture
Topics
Abstract EN
In vitro flowering and effective micropropagation protocol were studied in Swertia chirayita, an important medicinal plant using axillary bud explants.
The Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) 1.0 mg L-1 and adenine sulfate 70.0 mg L-1 was found optimum for production of multiple shoots.
In the present study, incubation of flowering cultures on BAP supplemented medium (during shoot multiplication) was found necessary for flowering (6 weeks).
However, concentrations of auxins-like IBA (0–2.0 mg/L) were ineffective to form reproductive buds.
Subculture duration, photoperiod, and carbon source type do have influence on the in vitro flowering.
The mature purple flowers were observed when the cultures were maintained in the same medium.
This is the very first report that describes in vitro flowering system to overcome problems associated with flower growth and development as well as lay foundation for fruit and seed production in vitro in Swertia chirayita.
American Psychological Association (APA)
Sharma, Vikas& Kamal, Barkha& Srivastava, Nidhi& Dobriyal, Anoop Kumar& Jadon, Vikash Singh. 2014. In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.. Biotechnology Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-458666
Modern Language Association (MLA)
Sharma, Vikas…[et al.]. In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.. Biotechnology Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-458666
American Medical Association (AMA)
Sharma, Vikas& Kamal, Barkha& Srivastava, Nidhi& Dobriyal, Anoop Kumar& Jadon, Vikash Singh. In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.. Biotechnology Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-458666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458666