Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules
Joint Authors
Liebscher, Jürgen
Kang, Jing
Kaczmarek, Oliver
Dähne, Lars
Source
International Journal of Polymer Science
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
H-aggregates of the cyanine dye Cy5 are formed during covalent linkage to the cationic macromolecule Poly(allylamine) (PAH).
The nonfluorescent H-aggregates strongly restrict the usage of the dye for analytical purposes and prevent a quantitative determination of the labeled macromolecules.
The behavior of the H-aggregates has been studied by investigation of the absorption and fluorescence spectra of the dye polymer in dependence on solvent, label degree and additional sulfonate groups.
H-aggregate formation is caused by an inhomogeneous distribution of the Cy5 molecules on the polymer chain.
The H-aggregates can be destroyed by conformational changes of the PAH induced by interactions with polyanions or in organic solvents.
It has been found that the polymer labeling process in high content of organic solvents can prevent the formation of H-aggregates.
The results offer a better understanding and improvement of the use of the Cy5 dye for labeling purposes in fluorescence detection of macromolecules.
American Psychological Association (APA)
Kang, Jing& Kaczmarek, Oliver& Liebscher, Jürgen& Dähne, Lars. 2010. Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules. International Journal of Polymer Science،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458673
Modern Language Association (MLA)
Kang, Jing…[et al.]. Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules. International Journal of Polymer Science No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-458673
American Medical Association (AMA)
Kang, Jing& Kaczmarek, Oliver& Liebscher, Jürgen& Dähne, Lars. Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules. International Journal of Polymer Science. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458673
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458673