Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo
Joint Authors
Dingman, Sherry
Gagnon, Zofia E.
Thomas, Rhys N.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Auxin a plant growth hormone, has a metabolic pathway that includes molecules and enzymes like those in animal brains.
In this study, tomato plant seedlings (Lycopersicon esculenta) were used to investigate the fate of fluorine-tagged 5-hydroxytryptophan (PF-5-HTP) being developed for fluorine spectroscopy and imaging.
Seedlings were treated with high or low concentrations of 5-HTP or PF-5-HTP and compared with controls.
Metabolites of the PF-5-HTP were quantified using a custom immunoassay for the tag.
Serotonin (5-HT) levels were measured with spectrofluorometry and thin-layer chromatography.
Plants in treatment conditions had serotonin levels five to six times higher than controls.
PF-5-HTP served as a precursor for serotonin in a biosynthetic pathway in this plant model, providing evidence for the bioavailability of the novel molecule.
The increase in serotonin in plants grown in media culture supplemented with 5-HTP or PF-5-HTP might have useful applications in pharmacology.
American Psychological Association (APA)
Gagnon, Zofia E.& Dingman, Sherry& Thomas, Rhys N.. 2010. Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo. Journal of Amino Acids،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458702
Modern Language Association (MLA)
Gagnon, Zofia E.…[et al.]. Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo. Journal of Amino Acids No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-458702
American Medical Association (AMA)
Gagnon, Zofia E.& Dingman, Sherry& Thomas, Rhys N.. Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo. Journal of Amino Acids. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-458702
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458702