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Interactions of Neuropathogenic Escherichia coli K1 (RS218) and Its Derivatives Lacking Genomic Islands with Phagocytic Acanthamoeba castellanii and Nonphagocytic Brain Endothelial Cells
Joint Authors
Yousuf, Zuhair
Khan, Hafsa
Iqbal, Junaid
Yousuf, Farzana Abubakar
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Here we determined the role of various genomic islands in E.
coli K1 interactions with phagocytic A.
castellanii and nonphagocytic brain microvascular endothelial cells.
The findings revealed that the genomic islands deletion mutants of RS218 related to toxins (peptide toxin, α-hemolysin), adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), protein secretion system (T1SS for hemolysin), invasins (IbeA, CNF1), metabolism (D-serine catabolism, dihydroxyacetone, glycerol, and glyoxylate metabolism) showed reduced interactions with both A.
castellanii and brain microvascular endothelial cells.
Interestingly, the deletion of RS218-derived genomic island 21 containing adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), protein secretion system (T1SS for hemolysin), invasins (CNF1), metabolism (D-serine catabolism) abolished E.
coli K1-mediated HBMEC cytotoxicity in a CNF1-independent manner.
Therefore, the characterization of these genomic islands should reveal mechanisms of evolutionary gain for E.
coli K1 pathogenicity.
American Psychological Association (APA)
Yousuf, Farzana Abubakar& Yousuf, Zuhair& Iqbal, Junaid& Siddiqui, Ruqaiyyah& Khan, Hafsa& Khan, Naveed Ahmed. 2014. Interactions of Neuropathogenic Escherichia coli K1 (RS218) and Its Derivatives Lacking Genomic Islands with Phagocytic Acanthamoeba castellanii and Nonphagocytic Brain Endothelial Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458737
Modern Language Association (MLA)
Yousuf, Farzana Abubakar…[et al.]. Interactions of Neuropathogenic Escherichia coli K1 (RS218) and Its Derivatives Lacking Genomic Islands with Phagocytic Acanthamoeba castellanii and Nonphagocytic Brain Endothelial Cells. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-458737
American Medical Association (AMA)
Yousuf, Farzana Abubakar& Yousuf, Zuhair& Iqbal, Junaid& Siddiqui, Ruqaiyyah& Khan, Hafsa& Khan, Naveed Ahmed. Interactions of Neuropathogenic Escherichia coli K1 (RS218) and Its Derivatives Lacking Genomic Islands with Phagocytic Acanthamoeba castellanii and Nonphagocytic Brain Endothelial Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-458737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458737