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In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy
Joint Authors
Morimoto, Yuhki
Uchida, Keiichi
Okugawa, Yoshinaga
Matsushita, Kohei
Kusunoki, Masato
Mizoguchi, Akira
Kawamura, Mikio
Araki, Toshimitsu
Inoue, Yasuhiro
Tanaka, Koji
Koike, Yuhki
Toiyama, Yuji
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research.
We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM).
We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months.
Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice.
First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse.
Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green).
In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation.
American Psychological Association (APA)
Tanaka, Koji& Morimoto, Yuhki& Toiyama, Yuji& Matsushita, Kohei& Kawamura, Mikio& Koike, Yuhki…[et al.]. 2011. In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy. Journal of Oncology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458742
Modern Language Association (MLA)
Tanaka, Koji…[et al.]. In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy. Journal of Oncology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-458742
American Medical Association (AMA)
Tanaka, Koji& Morimoto, Yuhki& Toiyama, Yuji& Matsushita, Kohei& Kawamura, Mikio& Koike, Yuhki…[et al.]. In Vivo Time-Course Imaging of Tumor Angiogenesis in Colorectal Liver Metastases in the Same Living Mice Using Two-Photon Laser Scanning Microscopy. Journal of Oncology. 2011. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-458742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458742