De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle
Joint Authors
Xu, Teng
Wang, Hui
Liu, Dongjun
Gao, Xiaoyu
Guo, Xudong
Du, Xiaoyuan
Source
International Journal of Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Despite that goat is one of the best nonmodel systems for villus growth studies and hair biology, limited gene resources associated with skin or hair follicles are available.
In the present study, using Illumina/Solexa sequencing technology, we de novo assembled 130 million mRNA-Seq reads into a total of 49,115 contigs.
Searching public databases revealed that about 45% of the total contigs can be annotated as known proteins, indicating that some of the assembled contigs may have previously uncharacterized functions.
Functional classification by KOG and GO showed that activities associated with metabolism are predominant in goat skin during anagen phase.
Many signaling pathways was also created based on the mapping of assembled contigs to the KEGG pathway database, some of which have been previously demonstrated to have diverse roles in hair follicle and hair shaft formation.
Furthermore, gene expression profiling of three skin types identified ~6,300 transcript-derived contigs that are differentially expressed.
These genes mainly enriched in the functional cluster associated with cell cycle and cell division.
The large contig catalogue as well as the genes which were differentially expressed in different skin types provide valuable candidates for further characterization of gene functions.
American Psychological Association (APA)
Xu, Teng& Guo, Xudong& Wang, Hui& Du, Xiaoyuan& Gao, Xiaoyu& Liu, Dongjun. 2013. De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-458989
Modern Language Association (MLA)
Xu, Teng…[et al.]. De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle. International Journal of Genomics No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-458989
American Medical Association (AMA)
Xu, Teng& Guo, Xudong& Wang, Hui& Du, Xiaoyuan& Gao, Xiaoyu& Liu, Dongjun. De Novo Transcriptome Assembly and Differential Gene Expression Profiling of Three Capra hircus Skin Types during Anagen of the Hair Growth Cycle. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-458989
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458989