Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives
Joint Authors
Martín-Orúe, Susana María
Pérez de Rozas, Ana María
Segalés, Joaquim
Pujols, Joan
Badiola, Ignacio
Castillo, Marisol
Majó, Natàlia
Nofrarías, Miquel
Espinal, Anna
Roca, Mercè
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
This study aimed to provide novel insights into the gastrointestinal microbial diversity from different gastrointestinal locations in weaning piglets using PCR-restriction fragment length polymorphism (PCR-RFLP).
Additionally, the effect of different feed additives was analyzed.
Thirty-two piglets were fed with four different diets: a control group and three enriched diets, with avilamycin, sodium butyrate, and a plant extract mixture.
Digesta samples were collected from eight different gastrointestinal segments of each animal and the bacterial population was analysed by a PCR-RFLP technique that uses 16S rDNA gene sequences.
Bacterial diversity was assessed by calculating the number of bands and the Shannon-Weaver index.
Dendrograms were constructed to estimate the similarity of bacterial populations.
A higher bacterial diversity was detected in large intestine compared to small intestine.
Among diets, the most relevant microbial diversity differences were found between sodium butyrate and plant extract mixture.
Proximal jejunum, ileum, and proximal colon were identified as those segments that could be representative of microbial diversity in pig gut.
Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.
American Psychological Association (APA)
Roca, Mercè& Nofrarías, Miquel& Majó, Natàlia& Pérez de Rozas, Ana María& Segalés, Joaquim& Castillo, Marisol…[et al.]. 2014. Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-459015
Modern Language Association (MLA)
Roca, Mercè…[et al.]. Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-459015
American Medical Association (AMA)
Roca, Mercè& Nofrarías, Miquel& Majó, Natàlia& Pérez de Rozas, Ana María& Segalés, Joaquim& Castillo, Marisol…[et al.]. Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-459015
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459015