AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells
Joint Authors
Minelli, Rosalba
Dianzani, Chiara
Cerbone, Angelo
Ferretti, Carlo
Barrera, Giuseppina
Roma, Guglielmo
Pizzimenti, Stefania
Ciamporcero, Eric Stefano
Canaparo, Roberto
Toaldo, Cristina
Dianzani, Mario Umberto
Pettazzoni, Piergiorgio
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
PPARαs are nuclear receptors highly expressed in colon cells.
They can be activated by the fibrates (clofibrate, ciprofibrate etc.) used to treat hyperlipidemia.
Since PPARα transcriptional activity can be negatively regulated by JNK, the inhibition of JNK activity could increase the effectiveness of PPARα ligands.
We analysed the effects of AS601245 (a JNK inhibitor) and clofibrate alone or in association, on proliferation, apoptosis, differentiation and the gene expression profile of CaCo-2 human colon cancer cells.
Proliferation was inhibited in a dose-dependent way by clofibrate and AS601245.
Combined treatment synergistically reduced cell proliferation, cyclin D1 and PCNA expression and induced apoptosis and differentiation.
Reduction of cell proliferation, accompanied by the modulation of p21 expression was observed in HepG2 cells, also.
Gene expression analysis revealed that some genes were highly modulated by the combined treatment and 28 genes containing PPRE were up-regulated, while clofibrate alone was ineffective.
Moreover, STAT3 signalling was strongly reduced by combined treatment.
After combined treatment, the binding of PPARα to PPRE increased and paralleled with the expression of the PPAR coactivator MED1.
Results demonstrate that combined treatment increases the effectiveness of both compounds and suggest a positive interaction between PPARα ligands and anti-inflammatory agents in humans.
American Psychological Association (APA)
Cerbone, Angelo& Toaldo, Cristina& Pizzimenti, Stefania& Pettazzoni, Piergiorgio& Dianzani, Chiara& Minelli, Rosalba…[et al.]. 2012. AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells. PPAR Research،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-459060
Modern Language Association (MLA)
Cerbone, Angelo…[et al.]. AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells. PPAR Research No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-459060
American Medical Association (AMA)
Cerbone, Angelo& Toaldo, Cristina& Pizzimenti, Stefania& Pettazzoni, Piergiorgio& Dianzani, Chiara& Minelli, Rosalba…[et al.]. AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells. PPAR Research. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-459060
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459060