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AKTmTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells
Joint Authors
Lin, Hung-Yun
Hsieh, Tze-Chen
Wu, Joseph M.
Lin, Chia-Yi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The polyphenol piceatannol has shown inhibition against tyrosine and serine/threonine kinases.
Whether piceatannol also exerts activity on the mammalian target of rapamycin (mTOR), a kinase involved in growth control of eukaryotic cells, is not known.
In this study, we tested the effects of piceatannol on proliferation of androgen-dependent (AD) LNCaP and androgen-independent (AI) DU145 and PC-3 prostate cancer (CaP) cells.
Suppression of AD and AI CaP cell growth by piceatannol was accompanied by cell cycle blockade in G1/S and S phases for LNCaP and PC-3 and induction of apoptosis in DU145 cells.
Induction of apoptosis by piceatannol in DU145 cells was evident by reduced expression of poly(ADP-ribose) polymerase (PARP), cleavage of caspase 3 and apoptosis inducing factor AIF, and an increase in cytochrome c.
The apoptotic changes occurred in concordance with DNA damage, supported by increased phosphorylated histone H2AX.
Immunoblot analyses showed that exposure of different-stage CaP cells to piceatannol also resulted in cell-type-specific downregulation of mTOR and its upstream and downstream effector proteins, AKT and eIF-4E-BP1.
We propose that the observed AKT and mTOR changes are new targets of piceatannol possibly contributing to its inhibitory activities on proliferation of CaP cells.
American Psychological Association (APA)
Hsieh, Tze-Chen& Lin, Chia-Yi& Lin, Hung-Yun& Wu, Joseph M.. 2012. AKTmTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells. ISRN Urology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459303
Modern Language Association (MLA)
Hsieh, Tze-Chen…[et al.]. AKTmTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells. ISRN Urology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-459303
American Medical Association (AMA)
Hsieh, Tze-Chen& Lin, Chia-Yi& Lin, Hung-Yun& Wu, Joseph M.. AKTmTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells. ISRN Urology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459303
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459303