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Potential Role of A2B Adenosine Receptors on ProliferationMigration of Fetal Endothelium Derived from Preeclamptic Pregnancies
Joint Authors
Escudero, Carlos
Troncoso, Felipe
Aguayo, Claudio
Salomon, Carlos
Sobrevia, Luis
Bertoglia, Patricio
Acurio, Jesenia
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To investigate the functionality of A2B adenosine receptor (A2BAR) and the nitric oxide (NO) and vascular endothelial growth factor (VEGF) signaling pathway in the endothelial cell proliferation/migration during preeclampsia, we used human umbilical vein endothelial cells (HUVECs) isolated from normal pregnancies n=15 or pregnancies with preeclampsia n=15.
Experiments were performed in presence or absence of the nonselective adenosine receptor agonist NECA, the A2BAR selective antagonist MRS-1754, and the nitric oxide synthase (NOS) inhibitor L-NAME.
Results indicated that cells from preeclampsia exhibited a significant higher protein level of A2BAR and logEC50 for NECA-mediated proliferation than normotensive pregnancies.
The stimulatory effect of NECA (10 μM, 24 h) on cell proliferation was prevented by MRS-1754 (5 nM) coincubation only in cells from normotensive pregnancies.
Nevertheless, L-NAME (100 μM, 24 h) reduced the NECA-induced cell proliferation/migration in HUVEC from normal pregnancy; however in preeclampsia only NECA-induced cell proliferation was reduced by L-NAME.
Moreover, NECA increased protein nitration and abundance of VEGF in cells from normal pregnancy and effect prevented by MRS-1754 coincubation.
Nevertheless, in preeclampsia NECA did not affect the protein level of VEGF.
In conclusion HUVECs from preeclampsia exhibit elevated protein level of A2BAR and impairment of A2BAR-mediated NO/VEGF signaling pathway.
American Psychological Association (APA)
Acurio, Jesenia& Troncoso, Felipe& Bertoglia, Patricio& Salomon, Carlos& Aguayo, Claudio& Sobrevia, Luis…[et al.]. 2014. Potential Role of A2B Adenosine Receptors on ProliferationMigration of Fetal Endothelium Derived from Preeclamptic Pregnancies. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-459462
Modern Language Association (MLA)
Acurio, Jesenia…[et al.]. Potential Role of A2B Adenosine Receptors on ProliferationMigration of Fetal Endothelium Derived from Preeclamptic Pregnancies. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-459462
American Medical Association (AMA)
Acurio, Jesenia& Troncoso, Felipe& Bertoglia, Patricio& Salomon, Carlos& Aguayo, Claudio& Sobrevia, Luis…[et al.]. Potential Role of A2B Adenosine Receptors on ProliferationMigration of Fetal Endothelium Derived from Preeclamptic Pregnancies. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-459462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459462