Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures
Joint Authors
Krueger, Alexander
Valtink, Monika
Roehlecke, Cora
Funk, Richard H. W.
Knels, Lilla
De la Vega Marin, Jamlec
Steiner, Gerald
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Water-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration.
We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs).
Nonirradiated and nonglyoxal-treated cells served as controls.
Experiments were carried out over a range of 37∘–45∘C with exact temperature monitoring to distinguish between temperature and wIRA effects.
Metabolic activity was assessed by resazurin assay.
Mitochondrial membrane potential was assessed by JC-1 vital staining.
Apoptotic changes were determined by vital staining with annexin V and YO-PRO-1 and determination of subG1 DNA content.
Temperature had a dominant effect overriding effects exerted by wIRA or glyoxal treatment.
The number of apoptotic cells was significantly higher at 45∘C, while the percentage of healthy cells was significantly lower at 45∘C.
WIRA irradiation itself or in combination with glyoxal treatment exerted no damaging effects on the fibroblasts at physiological (37∘–40∘C) or higher (42∘–45∘C) temperatures compared to untreated controls.
Temperatures of 45∘C, which can occur during inappropriate application of infrared irradiation, damage cells even in the absence of wIRA or glyoxal application.
American Psychological Association (APA)
Knels, Lilla& Valtink, Monika& De la Vega Marin, Jamlec& Steiner, Gerald& Roehlecke, Cora& Krueger, Alexander…[et al.]. 2012. Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459509
Modern Language Association (MLA)
Knels, Lilla…[et al.]. Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-459509
American Medical Association (AMA)
Knels, Lilla& Valtink, Monika& De la Vega Marin, Jamlec& Steiner, Gerald& Roehlecke, Cora& Krueger, Alexander…[et al.]. Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459509