Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use
Joint Authors
Bertozzi, Ezequiel
Frizzo, Laureano S.
Soto, Lorena P.
Rosmini, Marcelo R.
Avataneo, Elizabeth
Sequeira, Gabriel J.
Source
Veterinary Medicine International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The intestinal microbiota has an influence on the growth and health status of the hosts.
This is of particular interest in animals reared using intensive farming practices.
Hence, it is necessary to know more about complexity of the beneficial intestinal microbiota.
The use of molecular methods has revolutionized microbial identification by improving its quality and effectiveness.
The specific aim of the study was to analyze predominant species of Lactobacillus in intestinal microbial ecosystem of young calves.
Forty-two lactic acid bacteria (LAB) isolated from intestinal tract of young calves were characterized by: Amplified Ribosomal DNA Restriction Analysis (ARDRA), by using Hae III, Msp I, and Hinf I restriction enzymes, and 16S rDNA gene sequencing.
ARDRA screening revealed nine unique patterns among 42 isolates, with the same pattern for 29 of the isolates.
Gene fragments of 16S rDNA of 19 strains representing different patterns were sequenced to confirm the identification of these species.
These results confirmed that ARDRA is a good tool for identification and discrimination of bacterial species isolated from complex ecosystem and between closely related groups.
This paper provides information about the LAB species predominant in intestinal tract of young calves that could provide beneficial effects when administered as probiotic.
American Psychological Association (APA)
Soto, Lorena P.& Frizzo, Laureano S.& Bertozzi, Ezequiel& Avataneo, Elizabeth& Sequeira, Gabriel J.& Rosmini, Marcelo R.. 2009. Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use. Veterinary Medicine International،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-459515
Modern Language Association (MLA)
Soto, Lorena P.…[et al.]. Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use. Veterinary Medicine International No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-459515
American Medical Association (AMA)
Soto, Lorena P.& Frizzo, Laureano S.& Bertozzi, Ezequiel& Avataneo, Elizabeth& Sequeira, Gabriel J.& Rosmini, Marcelo R.. Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use. Veterinary Medicine International. 2009. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-459515
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459515