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Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin β Subunit to the N-Terminal and C-Terminal Coding Sequence
Joint Authors
Havron, Avri
Fares, Fuad
Fima, Eyal
Source
International Journal of Cell Biology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A new analog of EPO was designed by fusing one and two CTPs to the N-terminal and C-terminal ends of EPO (EPO-(CTP)3), respectively.
This analog was expressed and secreted efficiently in CHO cells.
The in vitro test shows that the activity of EPO-(CTP)3 in TFI-1 cell proliferation assay is similar to that of EPO-WT and commercial rHEPO.
However, in vivo studies indicated that treatment once a week with EPO-(CTP)3 (15 μg/kg) dramatically increased (~8 folds) haematocrit as it was compared to rHuEPO.
Moreover, it was found that EPO-(CTP)3 is more effective than rHuEPO and Aranesp in increasing reticulocyte number in mice blood.
The detected circulatory half-lives of rHuEPO, Aranesp, and EPO-(CTP)3 following IV injection of 20 IU were 4.4, 10.8, and 13.1 h, respectively.
These data established the rational for using this chimera as a long-acting EPO analog in clinics.
The therapeutic efficacy of EPO-CTP analog needs to be established in higher animals and in human clinical trials.
American Psychological Association (APA)
Fares, Fuad& Havron, Avri& Fima, Eyal. 2011. Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin β Subunit to the N-Terminal and C-Terminal Coding Sequence. International Journal of Cell Biology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-459521
Modern Language Association (MLA)
Fares, Fuad…[et al.]. Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin β Subunit to the N-Terminal and C-Terminal Coding Sequence. International Journal of Cell Biology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-459521
American Medical Association (AMA)
Fares, Fuad& Havron, Avri& Fima, Eyal. Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin β Subunit to the N-Terminal and C-Terminal Coding Sequence. International Journal of Cell Biology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-459521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459521