Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
Joint Authors
Mori, Shigeyasu
Rikimaru, Tetsuya
Hidaka, Saburo
Kaneko, Noriaki
Kaya, Hidehiro
Fujimura, Tetsuyuki
Source
International Journal of Dentistry
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration.
Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates.
Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured.
The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1.80±0.05 and 1.65±0.03, respectively.
These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration.
American Psychological Association (APA)
Kaneko, Noriaki& Rikimaru, Tetsuya& Fujimura, Tetsuyuki& Mori, Shigeyasu& Hidaka, Saburo& Kaya, Hidehiro. 2010. Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. International Journal of Dentistry،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-459528
Modern Language Association (MLA)
Kaneko, Noriaki…[et al.]. Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. International Journal of Dentistry No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-459528
American Medical Association (AMA)
Kaneko, Noriaki& Rikimaru, Tetsuya& Fujimura, Tetsuyuki& Mori, Shigeyasu& Hidaka, Saburo& Kaya, Hidehiro. Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. International Journal of Dentistry. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-459528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459528