Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes
Joint Authors
Sun, Li
Liu, Timon Cheng-Yi
Luo, Gang-Yue
Source
International Journal of Photoenergy
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-06
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability.
Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 μmol/L HgCl2, was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min.
Comprehensive morphological parameters of an intact cell such as contact area, perimeter, roundness and erythrocyte elongation index (EEI) were measured to characterize erythrocyte deformability with fast micro multi-channel spectrophotometer.
It was observed that the dosage of LHNL improvement of the morphological parameters of dehydrated erythrocytes was morphological-parameter-dependent, but the Bunsen-Roscoe rule did not hold for roundness.
The LHNL at 4.4 mW/cm2 for 5 min significantly improved the contact area (P<0.05) and EEI (P<0.05) of the dehydrated erythrocytes, but the improvement was significantly inhibited by 0.2 μmol/L HgCl2 (P<0.05).
It was concluded that AQP-1 might mediate the effects of LHNL on erythrocyte deformability, which supports the membranotropic mechanism of PBM.
American Psychological Association (APA)
Luo, Gang-Yue& Sun, Li& Liu, Timon Cheng-Yi. 2012. Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes. International Journal of Photoenergy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-459537
Modern Language Association (MLA)
Luo, Gang-Yue…[et al.]. Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes. International Journal of Photoenergy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-459537
American Medical Association (AMA)
Luo, Gang-Yue& Sun, Li& Liu, Timon Cheng-Yi. Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes. International Journal of Photoenergy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-459537
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459537