![](/images/graphics-bg.png)
The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy
Joint Authors
Xing, Tao
Cai, Xiaofan
Zeng, Rong
Zhao, Shilin
Zhu, Wenjie
Li, Qingrun
Li, Rongxia
Chen, Wanjia
Chen, Y. Eugene
Deng, Yueyi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Body fluid proteome is the most informative proteome from a medical viewpoint.
But the lack of accurate quantitation method for complicated body fluid limited its application in disease research and biomarker discovery.
To address this problem, we introduced a novel strategy, in which SILAC-labeled mouse serum was used as internal standard for human serum and urine proteome analysis.
The SILAC-labeled mouse serum was mixed with human serum and urine, and multidimensional separation coupled with tandem mass spectrometry (IEF-LC-MS/MS) analysis was performed.
The shared peptides between two species were quantified by their SILAC pairs, and the human-only peptides were quantified by mouse peptides with coelution.
The comparison for the results from two replicate experiments indicated the high repeatability of our strategy.
Then the urine from Immunoglobulin A nephropathy patients treated and untreated was compared by this quantitation strategy.
Fifty-three peptides were found to be significantly changed between two groups, including both known diagnostic markers for IgAN and novel candidates, such as Complement C3, Albumin, VDBP, ApoA,1 and IGFBP7.
In conclusion, we have developed a practical and accurate quantitation strategy for comparison of complicated human body fluid proteome.
The results from such strategy could provide potential disease-related biomarkers for evaluation of treatment.
American Psychological Association (APA)
Zhao, Shilin& Li, Rongxia& Cai, Xiaofan& Chen, Wanjia& Li, Qingrun& Xing, Tao…[et al.]. 2013. The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-459555
Modern Language Association (MLA)
Zhao, Shilin…[et al.]. The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-459555
American Medical Association (AMA)
Zhao, Shilin& Li, Rongxia& Cai, Xiaofan& Chen, Wanjia& Li, Qingrun& Xing, Tao…[et al.]. The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-459555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459555