Culture Models for Studying Thyroid Biology and Disorders
Joint Authors
Toda, Shuji
Matsunobu, Aki
Uchihashi, Kazuyoshi
Yamamoto, Mihoko
Yamasaki, Fumio
Sugihara, Hajime
Aoki, Shigehisa
Koike, Eisuke
Ootani, Akifumi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The thyroid is composed of thyroid follicles supported by extracellular matrix, capillary network, and stromal cell types such as fibroblasts.
The follicles consist of thyrocytes and C cells.
In this microenvironment, thyrocytes are highly integrated in their specific structural and functional polarization, but monolayer and floating cultures cannot allow thyrocytes to organize the follicles with such polarity.
In contrast, three-dimensional (3-D) collagen gel culture enables thyrocytes to form 3-D follicles with normal polarity.
However, these systems never reconstruct the follicles consisting of both thyrocytes and C cells.
Thyroid tissue-organotypic culture retains 3-D follicles with both thyrocytes and C cells.
To create more appropriate experimental models, we here characterize four culture systems above and then introduce the models for studying thyroid biology and disorders.
Finally, we propose a new approach to the cell type-specific culture systems on the basis of in vivo microenvironments of various cell types.
American Psychological Association (APA)
Toda, Shuji& Aoki, Shigehisa& Uchihashi, Kazuyoshi& Matsunobu, Aki& Yamamoto, Mihoko& Ootani, Akifumi…[et al.]. 2011. Culture Models for Studying Thyroid Biology and Disorders. ISRN Endocrinology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-459589
Modern Language Association (MLA)
Toda, Shuji…[et al.]. Culture Models for Studying Thyroid Biology and Disorders. ISRN Endocrinology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-459589
American Medical Association (AMA)
Toda, Shuji& Aoki, Shigehisa& Uchihashi, Kazuyoshi& Matsunobu, Aki& Yamamoto, Mihoko& Ootani, Akifumi…[et al.]. Culture Models for Studying Thyroid Biology and Disorders. ISRN Endocrinology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-459589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459589