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Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form
Joint Authors
Mahadik, Kakasaheb R.
Rathore, Atul S.
Sathiyanarayanan, L.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A simple, sensitive, precise, specific and stability indicating high-performance thin-layer chromatographic (HPTLC) method for the determination of emtricitabine both in bulk drug and pharmaceutical dosage form was developed and validated.
The method employed aluminium plates precoated with silica gel G60 F254 as the stationary phase.
The solvent system consisted of toluene : ethyl acetate : methanol (2 : 8 : 1, v/v/v).
This solvent system was found to give compact spots for emtricitabine with Rf value 0.26±0.01.
Densitometric analysis of emtricitabine was carried out in the absorbance mode at 284 nm.
Linear regression analysis showed good linearity (r2=0.9997) with respect to peak area in the concentration range of 30–110 ng spot-1.
The method was validated for precision, limit of detection (LOD), limit of quantitation (LOQ), robustness, accuracy and specificity.
Emtricitabine was subjected to acid and alkali hydrolysis, oxidation, neutral hydrolysis, photodegradation and dry heat treatment.
Also the degraded products peaks were well resolved from the pure drug with significantly different Rf values.
Statistical analysis proved that the method is repeatable and specific for the estimation of the said drug.
As the method could effectively separate the drugs from their degradation products, it can be employed as a stability indicating method.
American Psychological Association (APA)
Rathore, Atul S.& Sathiyanarayanan, L.& Mahadik, Kakasaheb R.. 2012. Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form. ISRN Chromatography،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-459748
Modern Language Association (MLA)
Rathore, Atul S.…[et al.]. Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form. ISRN Chromatography No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-459748
American Medical Association (AMA)
Rathore, Atul S.& Sathiyanarayanan, L.& Mahadik, Kakasaheb R.. Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form. ISRN Chromatography. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-459748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459748