Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes
Joint Authors
Lewko, Barbara
Latawiec, Elżbieta
Zieliński, Maciej
Pikuła, Michał
Rybczyńska, Apolonia
Maryn, Anna
Barczyńska, Anna
Source
Experimental Diabetes Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy.
In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells.
AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days.
Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity.
High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation.
Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR.
In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner.
Posttranslational events may affect AR activity independent of enzyme protein amount.
Activation of AR in podocytes may be implicated in diabetic podocytopathy.
American Psychological Association (APA)
Lewko, Barbara& Latawiec, Elżbieta& Maryn, Anna& Barczyńska, Anna& Pikuła, Michał& Zieliński, Maciej…[et al.]. 2011. Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes. Experimental Diabetes Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-459786
Modern Language Association (MLA)
Lewko, Barbara…[et al.]. Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes. Experimental Diabetes Research No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-459786
American Medical Association (AMA)
Lewko, Barbara& Latawiec, Elżbieta& Maryn, Anna& Barczyńska, Anna& Pikuła, Michał& Zieliński, Maciej…[et al.]. Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes. Experimental Diabetes Research. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-459786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459786