Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana
Joint Authors
Martínez Gómez, Federico
González Guzmán, Saúl
Barker, Douglas C.
Hernández-Montes, Omar
Monroy-Ostria, Amalia
Source
Interdisciplinary Perspectives on Infectious Diseases
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study analyzed DNA minicircles of Mexican isolates of L.
(Leishmania) mexicana to look for genetic differences between strains isolated from patients with diffuse cutaneous (DCL) and localized (LCL) leishmaniasis.
The kDNA was analyzed using polymerase chain reaction (PCR), restriction fragment polymorphism analysis of the PCR products (PCR-RFLP) and the PCR products were sequenced.
In the PCR with primers specific for the subgenus Leishmania, the Mexican isolates gave higher amplification products than the other L.
mexicana complex strains and with specific primers for the L.
mexicana complex they were poorly amplified.
In the PCR-RFLP analysis with the Eco RV, Hae III, and Mbo I endonucleases, the Mexican isolates displayed similar restriction patterns, but different from the patterns of the other members of the L.
mexicana complex.
In the phylogenetic tree constructed, the kDNA sequences of the Mexican clones formed two groups including sequences of LCD or LCL clones, apart from the other L.
mexicana complex members.
These results suggest that the kDNA minicircles of the Mexican isolates are more polymorphic than the kDNA of other members of the L.
mexicana complex and have different recognition sites for the restriction enzymes used in this study.
American Psychological Association (APA)
Hernández-Montes, Omar& González Guzmán, Saúl& Martínez Gómez, Federico& Barker, Douglas C.& Monroy-Ostria, Amalia. 2012. Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-459797
Modern Language Association (MLA)
Hernández-Montes, Omar…[et al.]. Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana. Interdisciplinary Perspectives on Infectious Diseases No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-459797
American Medical Association (AMA)
Hernández-Montes, Omar& González Guzmán, Saúl& Martínez Gómez, Federico& Barker, Douglas C.& Monroy-Ostria, Amalia. Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana. Interdisciplinary Perspectives on Infectious Diseases. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-459797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459797