The Adipocyte-Derived Hormone Leptin Has Proliferative Actions on Androgen-Resistant Prostate Cancer Cells Linking Obesity to Advanced Stages of Prostate Cancer
Joint Authors
Mohammed, Nasreldin
Hoda, M. Raschid
Fornara, Paolo
Theil, Gerit
Fischer, Kersten
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Because obesity may be a risk factor for prostate cancer, we investigated proliferative effects of adipocytes-derived hormone leptin on human prostate cancer cells and assessed the role of mitogen-activated protein kinase (MAPK) signaling pathway in mediating these actions.
Material and Methods.
Three human prostate cancer cell lines were treated with increasing doses of recombinant leptin.
Cell growth was measured under serum-free conditions using a spectrophotometric assay.
Further, Western blotting was applied to detect the phosphorylation of an ERK1/2, and a specific inhibitor of MAPK (PD98059; 40 μM) was used.
Results.
In both androgen-resistant cell lines DU145 and PC-3, cell growth was dose-dependently increased by leptin after 24 hrs and 48 hrs of incubation, whereas leptin’s proliferative effects on androgen-sensitive cell line LNCaP was less pronounced.
Further, leptin caused dose-dependent ERK1/2 phosphorylation in both androgen-resistant cell lines, and pretreatment of these cells with PD98059 inhibited these responses.
Conclusions.
Leptin may be a potential link between obesity and risk of progression of prostate cancer.
Thus, studies on leptin and obesity association to prostate cancer should differentiate patients according to androgen sensitivity.
American Psychological Association (APA)
Hoda, M. Raschid& Theil, Gerit& Mohammed, Nasreldin& Fischer, Kersten& Fornara, Paolo. 2012. The Adipocyte-Derived Hormone Leptin Has Proliferative Actions on Androgen-Resistant Prostate Cancer Cells Linking Obesity to Advanced Stages of Prostate Cancer. Journal of Oncology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459895
Modern Language Association (MLA)
Hoda, M. Raschid…[et al.]. The Adipocyte-Derived Hormone Leptin Has Proliferative Actions on Androgen-Resistant Prostate Cancer Cells Linking Obesity to Advanced Stages of Prostate Cancer. Journal of Oncology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-459895
American Medical Association (AMA)
Hoda, M. Raschid& Theil, Gerit& Mohammed, Nasreldin& Fischer, Kersten& Fornara, Paolo. The Adipocyte-Derived Hormone Leptin Has Proliferative Actions on Androgen-Resistant Prostate Cancer Cells Linking Obesity to Advanced Stages of Prostate Cancer. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-459895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459895