Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer
Joint Authors
El-Hussein, A.
Abrahamse, H.
Harith, M.
Source
International Journal of Photoenergy
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Photodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that localizes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light.
PDT rapidly induces cell death, inflammatory and immune reactions, and damage of the microvasculature.
DNA damage results from a variety of factors including UV-light, X-rays, ionizing radiation, toxins, chemicals, or reactive oxygen species.
The aim of this study was to determine the effect of PDT as well as the influence of presensitization leading to the adaptive response (AR) on the integrity of DNA.
Lung (A549), breast (MCF-7), and esophageal (SNO) cancer cells and Zn sulfophthalocyanine as PS with irradiation conditions of 10 J/cm2 at 636 nm were used.
Subcellular localization of PS, cell morphology, and viability after PDT and DNA damage were determined.
A significant decrease in viability and marked DNA damage was observed in all 3 cancer cell types in response to PDT while the adaptive response was demonstrated to significantly decrease the effectiveness of the PDT.
American Psychological Association (APA)
El-Hussein, A.& Harith, M.& Abrahamse, H.. 2012. Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer. International Journal of Photoenergy،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-459969
Modern Language Association (MLA)
El-Hussein, A.…[et al.]. Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer. International Journal of Photoenergy No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-459969
American Medical Association (AMA)
El-Hussein, A.& Harith, M.& Abrahamse, H.. Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer. International Journal of Photoenergy. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-459969
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459969