An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents
Joint Authors
Hall, Roy A.
Atwell, R. B.
Lee, R.
O'Donoghue, P.
Hall-Mendelin, S.
Source
Journal of Parasitology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The Ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in Australia.
Treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs.
Each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective.
With the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially purified antigen prepared from I.
holocyclus salivary glands to develop an ELISA to detect toxin-reactive antibodies in hyperimmune dog sera.
The optimised ELISA reliably detected antibodies reactive to I.
holocyclus salivary gland antigens.
Parallel testing of sera with a negative control antigen prepared from the salivary glands of the nontoxic tick Rhipicephalus (Boophilus) microplus provided further evidence that we were detecting toxin-specific antibodies in the assay.
Using the ELISA, we could also detect antibodies induced in rats after experimental infestation with I.
holocyclus.
This assay shows promise as an alternative means of assessing the potency of batches of hyperimmune dog serum and to screen for toxin-reactive monoclonal antibodies produced from immunised rodents.
American Psychological Association (APA)
Hall-Mendelin, S.& O'Donoghue, P.& Atwell, R. B.& Lee, R.& Hall, Roy A.. 2011. An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents. Journal of Parasitology Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-460180
Modern Language Association (MLA)
Hall-Mendelin, S.…[et al.]. An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents. Journal of Parasitology Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-460180
American Medical Association (AMA)
Hall-Mendelin, S.& O'Donoghue, P.& Atwell, R. B.& Lee, R.& Hall, Roy A.. An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents. Journal of Parasitology Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-460180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460180