Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNALNA Microarray
Joint Authors
Skreka, Konstantinia
Rederstorff, Mathieu
Scheideler, Marcel
Karbiener, Michael
Zywicki, Marek
Hüttenhofer, Alexander
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Mammalian transcriptomes mainly consist of non protein coding RNAs.
These ncRNAs play various roles in all cells and are involved in multiple regulation pathways.
More recently, ncRNAs have also been described as valuable diagnostic tools.
While RNA-seq approaches progressively replace microarray-based technologies for high-throughput expression profiling, they are still not routinely used in diagnostic.
Microarrays, on the other hand, are more widely used for diagnostic profiling, especially for very small ncRNA (e.g., miRNAs), employing locked nucleic acid (LNA) arrays.
However, LNA microarrays are quite expensive for high-throughput studies targeting longer ncRNAs, while DNA arrays do not provide satisfying results for the analysis of small RNAs.
Here, we describe a mixed DNA/LNA microarray platform, where directly labeled small and longer ncRNAs are hybridized on LNA probes or custom DNA probes, respectively, enabling sensitive and specific analysis of a complex RNA population on a unique array in one single experiment.
The DNA/LNA system, requiring relatively low amounts of total RNA, which complies with diagnostic references, was successfully applied to the analysis of differential ncRNA expression in mouse embryonic stem cells and adult brain cells.
American Psychological Association (APA)
Skreka, Konstantinia& Zywicki, Marek& Karbiener, Michael& Hüttenhofer, Alexander& Scheideler, Marcel& Rederstorff, Mathieu. 2012. Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNALNA Microarray. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460200
Modern Language Association (MLA)
Skreka, Konstantinia…[et al.]. Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNALNA Microarray. Journal of Nucleic Acids No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-460200
American Medical Association (AMA)
Skreka, Konstantinia& Zywicki, Marek& Karbiener, Michael& Hüttenhofer, Alexander& Scheideler, Marcel& Rederstorff, Mathieu. Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNALNA Microarray. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-460200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-460200